This is especially useful when large number of specimens needs to be cultured, as the instrument, which has been programmed for the same, automatically screens these. Dr.T.V.Rao MD 48.

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Dry 30-60 sec ( resource poor conditions ) Ideal to collect with al.

The finding of pathogenic microorganisms in a patients bloodstream is of great importance in terms of diagnosis, prognosis, and therapy.

L. Barth Reller, Clin.

Infect. Diseases, 1996 Dr.T.V.Rao MD 3.

It is employed to detect infections that are spreading through the bloodstream (such as bacteremia, septicemia amongst others).

This is possible because the bloodstream is usually a sterile environment Dr.T.V.Rao MD 4.

Subsequent identification of the microorganism and antibiotic susceptibility tests further guide effective antimicrobial therapy.

Bloodstream infection is the most severe form of infection and is frequently life-threatening, and blood culture to detect circulating microorganisms has been the diagnostic standard.

Dr.T.V.Rao MD 5.

Bact Alert 3D 60 Plus Clinical Signs

Meningitis, endocarditis) Septicemia bacteremia plus clinical signs and symptoms of bacterial invasion and toxin production Definitions Dr.T.V.Rao MD 6.

Dental procedures) Intermittent Can occur from abscesses at some body site that is seeding the blood Continuous Bacteremia Dr.T.V.Rao MD 9.

This procedure is performed by medical laboratory scientists, medical practitioners, some EMTs, paramedics phlebotomists and other nursing staff.

Venipuncture is one of the most routinely performed invasive procedures and is carried out for two reasons, to obtain blood for diagnostic purposes or to monitor levels of blood components (Lavery Ingram 2005).

Dr.T.V.Rao MD 17.

The key elements in labelling are: Patients surname, first and middle.

Patients ID number.

Date, time and initials of the phlebotomist must be on the label of EACH tube.

Bact Alert 3D 60 Verification Of The

Appropriate verification of the patients identity, by means of an armband or area specific procedure, will occur before the specimen collection.

Cultures should be drawn before administration of antibiotics, if possible.

Dry 30-60 sec ( resource poor conditions ) Ideal to collect with alcohol swabs containing 2 Chlorhexidine and 70 isopropyl alcohol Remove caps, clean with alcohol Put on gloves Without palpating, draw 20 ml and put 10 in anaerobic and 10 in aerobic bottle Dispose of syringe in sharps container Label bottles and send to lab Obtaining Blood Dr.T.V.Rao MD 27.

This requires that both the tops of the culture bottles and the venipuncture site of the patient are cleaned prior to collection with alcohol swabs containing 2 Chlorhexidine and 70 isopropyl alcohol.

Dr.T.V.Rao MD 28.

Using sterile gloves, do not wipe away the surgical solution, touch the puncture site, or in any way compromise the sterile process.

It is vital that the procedure is performed in as sterile a manner as possible as the persistent presence of skin commensals in blood cultures could indicate endocarditis but they are most often found as contaminants Dr.T.V.Rao MD 29.

Determine what tests are ordered and what tubes will be necessary BEFORE you begin to draw blood, and determine the order of draw for the tubes.

Dr.T.V.Rao MD 32.

Then remove the tube, and remove the needle.

Dr.T.V.Rao MD 33.

These conventional cultures using normal media take at least a minimum of 72 hours to isolate the pathogen and carry out susceptibility test to know the efficacy of antibiotics on simple aerobic bacteria.

This is especially useful when large number of specimens needs to be cultured, as the instrument, which has been programmed for the same, automatically screens these.

Dr.T.V.Rao MD 48.